
What You Can See
TRT touches more than testosterone, so Rythm watches the whole picture: total testosterone with calculated free testosterone (via SHBG and albumin), estradiol for conversion, SHBG itself (which often falls on TRT, changing your free-T dynamics), lipids and ApoB for the cardiovascular side, and hemoglobin with calculated hematocrit relevant because testosterone can stimulate red blood cell production. Kidney and liver markers round out the context.
Timing Is Everything
Consistency matters more than any single “perfect” time. Collect at the same point in your dosing interval each month many users test just before their next scheduled dose, or at another point chosen with their clinician. The one real mistake is alternating: a result near a post-dose peak should never be compared with one near the end of the interval, because the difference is pharmacology, not physiology. Follow your clinician's monitoring schedule; Rythm's morning at-home collection just makes sticking to it easy.
Avoiding a Contaminated Result
If you inject into the deltoid, use the opposite arm or place the device lower on the upper arm, away from the injection area. If you use topical testosterone, scrub the collection site with soap and water, dry with a clean paper towel, and keep gels or creams off the collection arm for at least 24 hours before testing residue can enter the sample directly and produce an artificially high result.
